人體雌激素對黑色素細胞的增生作用
Effects of estrogens on human melanocytes in vitro.
J Steroid Biochem Mol Biol. 1994 May;49(1):9-14.McLeod SD, Ranson M, Mason RS.
Department of Physiology, University of Sydney, New South Wales, Australia.
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Subjects with elevated serum estrogen concentrations, such as those who are pregnant or ingesting estrogen-containing contraceptive medication, may develop increased skin pigmentation. As little information is available on the mechanism(s) underlying this relationship, the in vitro effects of estrogens on melanocytes cultured from normal human skin were examined. Physiological concentrations of 17 beta-estradiol (10(-11) to 10(-9) M) significantly increased the activity of tyrosinase in melanocytes from 15 of 23 subjects. The observed increases ranged from 1.2- to 2.4-fold. Melanin synthesis, which correlated with tyrosinase activity (r = 0.98, P < 0.001) was increased to a similar extent. Melanin extrusion was also increased by 17 beta-estradiol (10(-9) M). The estrogens, estriol (10(-9) M) estrone (10(-9) M) stimulated tyrosinase activity melanin extrusion to a lesser extent than 17 beta-estradiol. The analogue 17 alpha-estradiol (10(-9) M) was shown to have effects on melanocyte tyrosinase activity melanin extrusion that were equivalent to those of 17 beta-estradiol. The pure estrogen antagonist ICI 164384 (10(-6) M) also stimulated tyrosinase activity. Cyclohexie (50 micrograms/ml) inhibited 17 beta-estradiol-induced tyrosinase stimulation (P < 0.001). These results indicate that several aspects of melanocyte function respond directly to estrogenic stimulation. The equivalent effects of the 17 alpha-analogue a "pure" anti-estrogen suggest that the 17 beta-estradiol response may be mediated through a non-classical mechanism which is similar to that described in other tissues of neural crest origin.
激素生化周刊，1994年5月
考克李德 教授， 蕓森 教授， 曼森 教授
生理醫(yī)學系，悉尼大學，澳大利亞
摘要
血液中雌激素較高的人群，譬如，懷孕婦女或使用含雌激素的避孕藥物的婦女，皮膚中色素含量會增加。針對雌激素與皮膚色素之間的反應(yīng)機制了解不多，本文針對該題目進行研究，現(xiàn)有23個受試者，其中15人體內(nèi)17beta雌三激素增加時，他們細胞內(nèi)鉻氨酸的量增加、活性增強時，黑色素隨之增多1.2至2.4倍。表皮黑色素的出現(xiàn)也同比增加。雌一、雌二激素對黑色素形成也會增加，但略少于17beta雌激素的影響。17Alpha雌激素對黑色素增加的程度與17beta雌三激素相似。非類固醇類的雌激素對黑色素有同樣增產(chǎn)的效果。蛋白合成抑制劑可以用來減低雌激素導致的黑色素增產(chǎn)，實驗結(jié)果是體內(nèi)雌激素會導致表皮黑色素增產(chǎn)。17beta雌三激素與17Alpha雌三激素及非類固醇雌激素對黑色素形成的影響是一樣的。
